Testing familial Relationships Using Simulated Blood test

Objective:
I did this hemotology lab in my genetics workshop. My objective was to find which parents; either Mr. and Mrs. Smith or Mr.and Mrs Jones were the biological parent, of either child one or child two.
Materials:
6 Blood typing slides
6 Simulated blood samples
(Mr. Smith)
(Mrs. Smith)
(Mr. Jones)
(Mrs. Jones)
(Child one)
(Child two)
Simulated Anti-A Serum
Simulated Anti-B Serum

Procedure (I will fill in later)

Scenario
Two children, born in a hospital at approximately the same time, may have been "mixed up" in the nursery. The parents of the children have volunteered to give blood samples to help determine whether or not this is the case.

Table # 1 (Agglutination Reaction)
Slide # and Name Anti-A Serum Anti-B Serum Blood type
1. Mrs. Smtih + - A
2. Mr. Jones + - A
3. Mrs. Jones - - O
4 . Mr. Smith + + AB
5. Child one + - B
6. Child two - - O

Table # 2 (Possible Genotypes)
Blood Type Possible Genotypes
Mr. Smith AB IA IB
Mrs. Smith A IA IA or IA i
Mr. Jones A IA IA or IA i
Mrs. Jones O IB IB or IB i
Child # 1 B IB IB or IB i
Child # 2 O ii

Based on the data we can conlude that M. and Mrs. Smith are the parents of Child # 2. Where as Mr. and Mrs. Jones are the biological parents of Child # 1.

Disinfectent prohibits growth of bacteria more effectively that anitseptic(iodine) and various antibiotics tested

My goal in this lab was to determine which of the folowing: Bleach disinfectant, iodine antiseptics or a number of various antibiotics most effectively prohibited the growth of the bacteria E.Coli.What you see in the above pictures are the pitri dishes I used to complete the experiment. My independant variable in the experiment was the antiseptic, antibiotics, and disinfectant. Where as my Dependant variable was my ZI (Zone of Inobition)

Turbidimetry determination of bacterial numbers

I spent about five hours on this paticular lab. When we ran this experiment we had to do a standard turbidity count. This vial contained a tryptic soy broth medium with no bacteria in it, which was used as a control.

Standard plate count test

In this experiment we grew E. Coli cultures in six different pitri dishes. Among the the four ml of agar present we mixed either 1ml or .1ml of diluted E.Coli bacteria

Bacteria staining and technique

Bacteria staining is a relativelaty intricate process that requires time and patience. It is not an exact science which allows for a number of possible mistakes and ambiguos results. Results from staining can reveal a great dea ofl information regarding the bacteria including;

1.Whether or not the paticular bacteria is gram negative or gram positiveunicellular organism

2.The relative complexity of the cell wall